Skip to main content
Skip to main menu Skip to spotlight region Skip to secondary region Skip to UGA region Skip to Tertiary region Skip to Quaternary region Skip to unit footer

Slideshow

Mitochondrial Fe-S cluster assembly in Arabidopsis thaliana

Tamanna Azam
Tamanna Azam
Department of Chemistry
University of Georgia
Chemistry Building, Room 400
Inorganic Seminar

In plants, three Fe-S cluster assembly pathways exist namely SUF, ISC and CIA, involved in the maturation of iron sulfur proteins in the plastid, mitochondria and cytosol respectively. In this talk, we report on anaerobic purification of three classes of recombinant mitochondrial Fe-S cluster carrier proteins from A. thaliana, namely GRXS15, ISCA1a/2, and NFU4/5, and characterization of their Fe-S cluster content using UV-visible absorption/CD, resonance Raman, EPR, and analytical studies. GRXS15 contained trace amounts of [2Fe-2S] clusters as purified and cluster reconstitution in the presence of GSH resulted in a homogeneous [2Fe-2S] cluster-bound form. Co-expression of ISCA1a/2 resulted in samples with one [2Fe-2S] cluster per heterodimer. Unlike [2Fe-2S] cluster-bound Azotobacter vinelandii NifIscA homodimer (1), [2Fe-2S] cluster-bound ISCA1a/2 did not undergo reversible conversion to a [4Fe-4S] cluster-bound form on addition of DTT. However, cluster reconstitution using the as purified [2Fe-2S] cluster-bound ISCA1a/2 heterodimer did result in a predominantly [4Fe-4S] cluster-bound form of the ISCA1a/2 heterodimer. NFU4 and NFU5 were both purified as apo proteins, but both assembled one [4Fe-4S] cluster per homodimer after cluster reconstitution. In order to establish the role of these proteins in plant mitochondrial Fe-S cluster trafficking, cluster transfer reactions were monitored by UV-visible absorption and CD spectroscopy. The major conclusions thus far are that [2Fe-2S] cluster-bound GRXS15 is competent for assembling [4Fe-4S] clusters on the ISCA1a/2 heterodimer and [4Fe-4S]-ISCA1a/2 can transfer a [4Fe-4S] cluster to the NFU4 and NFU5 proteins. However, [2Fe-2S] cluster-bound ISCA1a/2 is not competent for the assembly of either [2Fe-2S] or [4Fe-4S] clusters on NFU4 or NFU5, and [4Fe-4S] cluster-bound NFU4 and NFU5 are not competent for the maturation of mitochondrial aconitase.

Reference

  1. Mapolelo, D. T., Zhang, B., Naik, S. G., Huynh, B. H., and Johnson, M. K. (2012) Spectroscopic and functional characterization of iron-bound forms of Azotobacter vinelandii (Nif)IscA. Biochemistry 51, 8071-8084

Support Us

We appreciate your financial support. Your gift is important to us and helps support critical opportunities for students and faculty alike, including lectures, travel support, and any number of educational events that augment the classroom experience. Click here to learn more about giving.

Every dollar given has a direct impact upon our students and faculty.

Got More Questions?

Undergraduate inquiries: chemreg@uga.edu 

Registration and credit transferschemreg@uga.edu

AP Credit, Section Changes, Overrides, Prerequisiteschemreg@uga.edu

Graduate inquiries: chemgrad@uga.edu

Contact Us!

Assistant to the Department Head: Donna Spotts, 706-542-1919 

Main office phone: 706-542-1919 

Main Email: chem-web@franklin.uga.edu

Head of Chemistry: Prof. Jason Locklin