Chemistry Faculty:
I. Jonathan Amster, Ph.D.
Professor and Associate Head
Phone: 706-542-2001
E-mail: jamster@uga.edu
Biographical Information
Ph.D., Cornell University, 1986
Research Interests
My research group specializes in the development and application of Fourier transform mass spectrometry for solving difficult problems in bioanalytical chemistry. Our research efforts are principally focused in the area of proteomic analysis. We are developing rapid and sensitive methods for measuring changes in the cellular levels of proteins. Our approach is to harvest complex protein mixtures from a cell, enzymatically digest the proteins into peptides, and to identify the peptides by accurate mass measurement using Fourier transform mass spectrometry. This process is aided by a combination of capillary scale separation methods, and chemical derivitization methods using compounds synthesized in our laboratory. Other recent research projects include the high resolution analysis of biological molecular aggregates, the mass spectrometric characterization of metalloproteins, structural studies of synthetic polymers, and the development of FTMS techniques for ion remeasurement and tandem mass spectrometry of macromolecules.
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Publications
Ding. W.; Johnson, K. A.; Kutal, C.; Amster, I. J., “Mechanistic Studies of Photochemical Reactions with Millisecond Time Resolution by Electrospray Ionization Mass Spectrometry”, Anal. Chem. 2003, 75, 4624-4630.
Ding, W.; Sanderson, C. T.; Conover, R. C.; Johnson, M. K.; Amster, I. J.; Kutal, C., “Characterization of the Low-Energy Electronic Excited States of Benzoyl-Substituted Ferrocenes”, Inorg. Chem. 2003, 42, 1532-1537.
Taylor, P. K.; Amster, I. J., “Space Charge Effects on Mass Accuracy for Multiply Charged Ions in ESI-FTICR”, Int. J. Mass Spectrom. 2003, 222, 351-361.
Sanderson, C. T.; Palmer, B. J.; Morgan, A.; Murphy, M.; Dluhy, R. A.; Mize, T.; Amster, I. J.; Kutal, C., “Classical Metallocenes as Photoinitiators for the Anionic Polymerization of an Alkyl 2-CyanoAcrylate”, Macromolecules 2002, 35, 9648-9652.
Turner, C. A.; Ding, W; Amster, I. J.; Kutal, C. R., “Ground- and Excited-State Reactivities of Cationic Sandwich and Half-Sandwich Complexes of Iron (II)”, Coord. Chem. Rev. 2002, 229, 9-16.
Ding, W.; Johnson, K. A.; Amster, I. J.; Kutal, C., “Identification of Photogenerated Intermediates by Electrospray Ionization Mass Spectrometry: New Insights Concerning the Photochemistry of CpFe(η6-arene)+ (Cp is η5-cyclopentadienyl) and Its Role as a Photoinitiator for Epoxide Polymerization”, Inorg. Chem. 2001, 40, 6865-66.
Smith, A. D.; Agar, J. N.; Johnson, K. A.; Frazzon, J.; Amster, I. J.; Dean, D. R.; Johnson, M. K., “Sulfur Transfer from IscS to IscU. The First Step in Iron-Sulfur Cluster Biosynthesis”, J. Am. Chem. Soc. 2001, 123, 11103-11104.
Johnson, K. A.; Brereton, P. S.;Verhagen, M. F. J. M.; Calzolai, L.; La Mar, G. N.; Adams, M. W. W.; Amster, I. J., “A Gallium-Substituted Cubane-Type Cluster in Pyrococcus furiosus Ferredoxin”, J. Am. Chem. Soc. 2001, 123, 7935-7936.
Taylor, P. K., Kurtz, D. M., Jr.; Amster, I. J., “Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Multimeric Metalloproteins”, Int. J. Mass Spectrom. 2001, 210-211, 651-663.
Johnson, K. A.; Amster, I. J., "First Observation by Mass Spectrometry of a 3+ Oxidation State for a [4Fe-4S] Metalloprotein: An ESI-FTICR Mass Spectrometry Study of the High Potential Iron-Sulfur Protein from Chromatium vinosum" J. Am. Soc. Mass Spectrom. 2001, 12, 819-825.
Johnson, K. A.; Shira, B. A.; Anderson, J. L.; Amster, I. J., "Chemically and On-Line Electrochemically Reduced Metalloprotein Studies by High Resolution Electrospray Ionization Mass Spectrometry," Anal. Chem. 2001, 73, 803-808.
Taylor, P. K.; Parks, B. A.; Kurtz, Jr., D. M.; Amster, I. J., "Analysis of Metal Incorporation During Overexpression of Clostridium Pasteurianum Rubredoxin by Electrospray FTICR Mass Spectrometry" J. Biol. Inorg. Chem. 2001, 6, 201-206.
Johnson, K. A.; Verhagen, F. J. M.; Adams, M. W. W.; Amster, I. J., "Differences Between Positive and Negative Ion Stabilities of Metal-Sulfur Cluster Proteins: an ESI FTICR Study" Int. J. Mass Spectrom. 2001, 204, 77-85.
Mize, T. H.; Amster, I. J., "Broadband Ion Accumulation with an Internal Source MALDI-FTICR Mass Spectrometer" Anal. Chem. 2000, 72, 5886-5891.
Johnson, K. A.; Verhagen, M. F. J. M.; Brereton, P. S.; Adams, M. W. W.; Amster, I. J., "Probing the Stoichiometry and Oxidation States of Metal Centers in Iron-Sulfur Proteins Using Electrospray FTICR Mass Spectrometry" Anal. Chem. 2000, 72, 1410-1418.