Molecular Genetics Instrumentation Facility

The MGIF comprises two basic service labs, one dealing with proteins and the other with DNA. The first analyzes and synthesizes proteins and peptides and gives researchers details about their samples. For protein sequencing, the facility has a new ABI Procise (494) protein sequencer. This instrument can sequence using either gas and pulsed liquid chemistry, and allows us to choose the best chemistry for your particular needs. The MGIF also provides peptide synthesis, and has an Advanced Chemtech Model 350 synthesizer. The 350 can synthesize up to 35 peptides simultaneously. A new option is the ability to synthesize multiple antigenic peptides, consisting of either four or eight copies of the peptide covalently bound to a poly-lysine core.

The other MGIF lab concentrates on DNA analysis and synthesis. For DNA synthesis, MGIF has two four-column instruments with a potential output of 18 oligonucleotides (oligos) a day. Turnaround time is 24-48 hours for an average oligo. The facility also has the ability to make oligos incorporating non-standard bases. A recent addition to our services is DNA sequencing on an automated fluorescence-based sequencer. The MGIF can sequence single stranded DNA, double stranded DNA, or PCR products. Data is provided as a color printout, and the sequence is e-mailed directly to your network account in GCG format. With a total of 4 sequencers the turnaround time is four to five working days.

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